OPEN see commentary on page 265 Noninvasive diagnosis of primary membranous nephropathy using phospholipase A2 receptor antibodies Shane A. Bobart1,4, An S. De Vriese2,4, Aditya S. Pawar1, Ladan Zand1, Sanjeev Sethi3, Callen Giesen3, John C. Lieske1,3 and Fernando C. Fervenza1 1Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA; 2Division of Nephrology, AZ … A titer increase, decrease, or disappearance generally precedes a change in clinical status. 6. 1 It is used as an aid in the diagnosis of primary membranous glomerulonephritis (pMGN), in conjunction with other laboratory and clinical findings. Beck LH, Bonegio RGB, Lambeau GR, et al. N Engl J Med. This test aids in the differential diagnosis of membranous glomerulonephritis (MGN) or nephrotic syndrome of unknown etiology. Additional charges apply.

[1,2,3,4] Idiopathic membranous nephropathy (iMN) has been found of having an immune etiologic basis.Various data such as kidney biopsy findings on light microscopy, immunofluorescence staining for granular immunoglobulin G (mainly IgG4) and C3 deposition along … Anti-neutrophil cytoplasmic antibody-associated glomerulonephritis with detection of myeloperoxidase and phospholipase A 2 receptor in membranous nephropathy-lesions: report of two patients with microscopic polyangiitis Kenta Tominaga1*, Takahiro Uchida1, Toshihiko Imakiire1, Kenji Itoh2, Hideyuki Shimazaki3, Kuniaki Nakanishi3, Qin W, Beck LH Jr, Zeng C, et al. 12 A recent study showed that 70% of patients with an apparently idiopathic membranous nephropathy had autoantibodies to the M-type phospholipase A2 receptor (anti-PLA2R), which is found in podocytes. Introduction . Membranous nephropathy (MN) accounts for most cases of the nephrotic syndrome in adults. Therapy outcome can be monitored by measuring the anti-phospholipase A2 receptor (PLA2R) antibody titer. The receptor under normal circumstances binds PLA2, but its exact physiological role is unclear . A positive result (1:10 or greater) for phospholipase A2 receptor antibody, IgG, in conjunction with other laboratory and clinical findings, supports a diagnosis of primary membranous glomerulonephritis … Kidney Int Suppl. Circulating autoantibodies against M-type phospholipase A2 receptor (PLA2R) are important pathogenic antibodies of … These include systemic lupus erythematosus, malignancy (particularly in patients aged over 65) and hepatitis B infection. Phospholipase A2 receptor (PLA2R) is present in normal podocytes and in immune deposits in patients with idiopathic membranous nephropathy, indicating that it is a major antigen in this disease. identified a circulating antibody reactive with the transmembrane glycoprotein M-type phospholipase A 2 receptor (PLA 2 R) on the human podocyte. Phospholipase A2 receptor (PLA2R) is present in normal podocytes and in immune deposits in patients with idiopathic membranous nephropathy, indicating that it is a major antigen in this disease. Monospecific detection of antibodies against phospholipase A2 receptor (PLA2R). PLA2R : Membranous nephropathy (MN) is a rare disease in which immune complexes deposit at the glomerular basement membrane, causing damage to the filtration barrier, resulting in proteinuria. Kidney biopsy is the gold standard to diagnose membranous nephropathy (MN). Anti-phospholipase A 2 receptor antibody in membranous nephropathy. Beck LH Jr, Bonegio RG, Lambeau G, Beck DM, Powell DW, Cummins TD, Klein JB, Salant DJ. Membranous glomerulonephritis (MGN) can be caused a by It has been demonstrated that antibodies to PLA 2 R (anti-PLA 2 R) are very specific for iMN, with anti-PLA 2 R detected in up to 82% of patients. To discriminate between different forms of nephritis without having to perform a kidney biopsy, new serologic biomarkers are needed. Anti-Phospholipase A2 Receptor Antibody Positive Primary Membranous Glomerulonephritis with Partial Remission of Proteinuria and Complete Disappearance of Antibody Marker with Rituximab Therapy Ramy M. Hanna, MD, Andrae Vandross, MD, and Rumi Cader, MD. In systemic lupus erythematosus (SLE), the most common and severe forms of lupus nephritis are classified as … 2012;2(2):186-197. Antigen: recombinant, human cDNA expressed in a human cell line. We hypothesized that PLA2R antibody testing without kidney biopsy may be a valid strategy to make a non-invasive diagnosis of MN in patients with a negative work-up for secondary causes. The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. The anti-PLA2R ELISA (IgG) test kit is intended for the qualitative or semiquantitative determination of IgG class autoantibodies against phospholipase A2 receptor (PLA2R) in human serum. Chapter 7: idiopathic membra-nous nephropathy. Thus, the determination of the antibody titer has a high predictive value with respect to clinical remission, relapse, or risk assessment after kidney transplantation. Note: If Phospholipase A2 Receptor Antibody, IgG is positive, then a Phospholipase Receptor A2 Antibody, IgG titer is reported.